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PrimerSuite
Type or paste (Ctrl-V) sequence(s) of the templates here in FASTA format here
Or upload a fasta file:
Or download a copy of sample sequences
here
and upload the file for primer design
Bisulfite
Genomic
Repbase check
Report Best primers only
PRIMER DESIGN PARAMETERS
FORWARD
REVERSE
CpG's in Oligo
Location of CpGs
Fusion sequences
MINIMIUM
MAXIMIUM
GC content (%) (gDNA only)
Amplicon Size (bp)
Oligo melting temp (°C)
Min Dimer Score Cutoff
✪
Sodium Concentration (mM)
Min CpGs in amplicon (bisulfite-only)
Primer Score (bisulfite-only)
homopolymer(s)